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Picture of Bengal Bioscience Home/Office Saliva Testing Kits for Growth Factors and Cytokines. We Test For Saliva Growth Factors 
In Support of Stem Cell Therapeutics. Prove the Efficacy of Your Therapies
And Experimental Trials
With More Than Reliance On Only Symptoms. With Our Home/Office Saliva Test Kits

Now You Can Prove The Efficacy of Your Therapies or Experimental Trials, Regardless of Whether They Might Be:

–stem cell therapy via mini-liposuction of abdominal tissue (the Stromal Vascular Fraction-SVF)

—IV stem cell therapy using umbilical cord stem cells

–stem cell therapy without the stem cells via high dose, light-activated, platelet rich plasma (PRP)

–endogenous stimulation or activation of stem cells via supplements

–endogenous stimulation or activation of stem cells via Low Level Laser Therapy (LLLT) applied to the abdomen

–endogenous stimulation or activation of stem cells via lipolysis of abdominal fatty tissue via the use of injected lipolytic agents designed to break up the adipose tissue matrix

–any other therapeutic application that may be of interest

Bengal Bioscience, led by stem cell scientist Walter P. Drake, has developed proprietary technology and protocols, that allow for affordable patient saliva testing before and after a procedure. The Company provides kits containing collection tubes and pre-paid mailer for saliva to be collected and sent in for testing.

A stem cell scientist for 25 years, Dr. Drake has been able to resurrect the old Ouchterlony immunodiffusion technique, by which antibody directed Bengal Bioscience, led by stem cell scientist Walter P. Drake, has developed proprietary technology and protocols, that allow for affordable patient saliva testing before and after a procedure. The Company provides kits containing collection tubes and pre-paid mailer for saliva to be collected and sent in for testing. A stem cell scientist for 25 years, Dr. Drake has been able to resurrect the old Ouchterlony immunodiffusion technique, by which antibody directed to a growth factor or other cytokine, precipitates as antigen-antibody complexes in agar gel. Due to modern computer and software technology, these bands of precipitation can then be quantified, yielding important information about the success of a stem cell treatment. Most importantly, by making use of this old yet upgraded technology, the testing of a selection of important growth factors can be done at an affordable cost, estimated at $500-$750. Keeping costs down is extremely important because these procedures are not covered by health insurance and require the patient to pay for each procedure. to a growth factor or other cytokine, precipitates as antigen-antibody complexes in agar gel. Due to modern computer and software technology, these bands of precipitation can then be quantified, yielding important information about the success of a stem cell treatment. Most importantly, by making use of this old yet upgraded technology, the testing of a selection of important growth factors can be done at an affordable cost, estimated at $500-$750. Keeping costs down is extremely important because these procedures are not covered by health insurance and require the patient to pay for each procedure.

Bengal Bioscience is a research and development laboratory with proprietary technology and protocols for testing growth factors and cytokines in saliva before and after a stem cell  therapeutic.

There is currently little to no testing of any kind to demonstrate whether a stem cell treatment modality has been successful, or caused any beneficial effect on the patient. Most if not all tests are cost prohibitive.

All About Our Testing

The Procedure: Saliva Collection and Preliminary Processing. Saliva is collected by the patient or health care provider/clinical scientist via the Bengal Bioscience home test kit. The passive procedure is used wherein 3-5 ml of passive drool is collected into a test tube for sending to our lab. Saliva testing is now commonplace, with labs such as ZRT Labs using saliva test kits to measure hormones. ZRT Labs Saliva Test Kit

The saliva kit is received at our lab, and the saliva sample is centrifuged to remove any particulate matter, and made ready for testing.

Our Methodology. Samples are then challenged with antibody to various growth factors and cytokines using the Ouchterlony double-diffusion assay, which is an immunochemistry test. Quantification is obtained via microscopic image acquisition using phase contrast and/or immunofluorescence microscopy; followed by software based image analysis for quantification. Comparison of intensities are thereby achieved objectively and without technician variability.

Our reagents. Although growth factor substrates are used to create our antisera, we make our very important antisera to various growth factors ourselves. We send the antigens to our few domestic providers who house our rabbits, and who, under our specifications, create our antisera. After conjugating with fluorescent tags, if appropriate, we verify activity and use our reagents in our testing. And we are developing an inventory of antisera to MOST of the saliva growth factors, not just to 2-3 items as with most clinical blood testing labs.

Our Results: The amount of  antigen/antibody complexes formed in bands in the agar gel, is measured as intensity of imaging. The intensity data goes to a chart, and then using charting software, the data is converted to a bar graph for presentation of results. A typical run when using Fluorescein conjugated antibody looks like this:

Bengal Bioscience Flow Diagram of Ouchterlony Assay Results using Fluorescein conjugated antibody. The Procedure: Saliva Collection and Preliminary Processing. Saliva is collected by the patient or health care provider/clinical scientist via the Bengal Bioscience home test kit. The passive procedure is used wherein 3-5 ml of passive drool is collected into a test tube for sending to our lab. The saliva kit is received at our lab, and the saliva sample is centrifuged to remove any particulate matter, and made ready for testing. Our Methodology. Samples are then challenged with antibody to various growth factors and cytokines using the Ouchterlony double-diffusion assay, which is an immunochemistry test. Quantification is obtained via microscopic image acquisition using phase contrast and/or immunofluorescence microscopy; followed by software based image analysis for quantification. Comparison of intensities are thereby achieved objectively and without technician variability.

Absolute Concentration of Growth Factor Is Not Needed,
only the extent of change.

It should be emphasized that we are not seeking absolute concentration of a growth factor in a patient’s saliva. This would be an irrelevant number, because there is a wide variation of base amounts in a patient population due to age of the patient, genetic makeup, and disease status. The most important information is what happens to some growth factor before and after a therapeutic event. The patient serves as his/her own control. Did, for example, high dose PRP therapy improve growth factor concentrations in the patient after, compared to before?

Your Cost. Your cost will vary between $500-$750 depending on the reagents involved and what growth factors and/or cytokines you are testing. Because we are never concerned with knowing absolute concentration, every test will typically require at least 2 before and after samples.  We provide the kit to you or your patient. Different from hormone testing, it does not do a patient any good to know anything about their growth factors by themselves without any therapeutic trial or event. We supply the saliva collection and return kits by mail.

Bengal Bioscience testing reagents. Although growth factor substrates are used to create our antisera, we make our very important antisera to various growth factors ourselves. We send the antigens to our few domestic providers who house our rabbits, and who, under our specifications, create our antisera. After conjugating with fluorescent tags, if appropriate, we verify activity and use our reagents in our testing. And we are developing an inventory of antisera to MOST the saliva growth factors, not just to 2-3 items as with most clinical blood testing labs. Our Results: The amount of antigen/antibody complexes formed in bands in the agar gel, is measured as intensity of imaging. The intensity data goes to a chart, and then using charting software, the data is converted to a bar graph for presentation of results. A typical sample results bar graph looks like this:

Disclaimer and Important Notices
Research Purposes ONLY, Not for Clinical Use or Clinical Diagnosis. Our Saliva Growth Factor and Cytokine Testing is intended ONLY for research purposes and for use in evaluating various therapeutic modalities in a clinical research environment. These tests cannot be used to diagnose any disease or condition, and are intended to be used only by health care practitioners and clinical scientists in evaluating research results.
Not Approved by FDA. Our tests are not approved by the FDA, and do not qualify for any insurance payments. The FDA and various States only approve clinical labs for clinical testing in the diagnosis of disease.
USA Customers Only. Due to the degradation of saliva samples, we can only serve customers in the continental United States. No Hawaii, Alaska, Puerto Rico, or customers in foreign countries can be serviced. Please do not send any funds, there are no refunds.